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    Vector Biolabs aav8 cmv null vector biolabs
    Aav8 Cmv Null Vector Biolabs, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav8+cmv+null+vector+biolabs/AAV8-Null/pm31056284-256-146-148
    Average 95 stars, based on 18 article reviews
    aav8 cmv null vector biolabs - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.

    Western Blot:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.

    Virus:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.

    Mutagenesis:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.

    Recombinant:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.

    Blocking Assay:

    Article Title: Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-GPX4 (Immunofluorescence) Lifespan Biosciences Cat#LS-B14362 Rabbit anti-GPX4 (Immunoblot) Lifespan Biosciences Cat#LS-B1596; RRID:AB_2263528 Rabbit anti-Sp1 EMD Millipore Cat#07-645; RRID:AB_310773 Mouse anti-NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Mouse anti-TFAP2c Santa Cruz Cat#SC12762; RRID:AB_667770 Rabbit Igg Santa Cruz Cat#SC2027; RRID:AB_737197 Mouse anti-TFAP2d Abcam Cat#ab169153 Rabbit anti-TFAP2a Cell Signaling Technology Cat#3208; RRID:AB_2056341 Rabbit anti-Histone H3 Cell Signaling Technology Cat#4499; RRID:AB_10544537 Mouse anti-Histone H3 Cell Signaling Technology Cat#3638; RRID:AB_1642229 Mouse anti-Histone H4 Cell Signaling Technology Cat#2935; RRID:AB_1147658 Rabbit anti-COX 4 Cell Signaling Technology Cat#4850; RRID:AB_2085424 Bacterial and Virus Strains DH5a Invitrogen Cat#18265-017 High efficiency 5a E.coli New England Bioscience Cat#C29871 Adenoviral ddGPX4 This paper/viraquest N/A Adenoviral Sp1 Ryu et al., 2003 N/A Adenoviral mutant Sp1 This paper/viraquest N/A Adenovirus GPX4-L D. Spitz/Viraquest N/A Adenovirus CAT This paper/viraquest N/A Adenovirus SOD2 This paper/viraquest N/A Adenovirus TFAP2c Kulak et al., 2013 N/A AAV8-eGFP Vector Biolabs Cat# 7061 AAV8-CMV Null Vector Biolabs Cat# 7077 AAV8-esyn-GPX4 This paper/Vector Biolabs N/A AAV8-esyn-mSp1 (mutant Sp1) This paper/Vector Biolabs N/A Chemicals, Peptides, and Recombinant Proteins Sodium Selenite Sigma Aldrich Cat#S5261 Collagenase Type VII-S Sigma Aldrich Cat#C2399 ToPro-3 Iodide (642/661) Invitrogen Cat#T3605 Fluoro Jade B EMD Milipore Cat#AG 310 MK801 Sigma Aldrich Cat#M107 Hemin Sigma Aldrich Cat#H9039 Actinomycin-D Sigma Aldrich Cat#A1410 L-homocysteic acid Sigma Aldrich Cat#H9633 Erastin Selleck Chemicals Cat#S7424 Trimethoprim (TMP) Sigma Aldrich Cat#T7883 N-acetyl-cysteine Sigma Aldrich Cat#A7250 FIN56 Sigma Aldrich Cat#SML1740 Nonidet P40 Substitute Affymetrix Cat#9002-93-1 Thapsigargin Sigma Aldrich Cat#T9033 Tunicamycin Sigma Aldrich Cat# T-7765 1-hydroxy-7-azabenzotriazole (HOAt) Advanced ChemTech Cat# RC8208 (Continued on next page) Cell 177, 1–18.e1–e11, May 16, 2019 e1 Please cite this article in press as: Alim et al., Selenium Drives a Transcriptional Adaptive Program to Block Ferroptosis and Treat Stroke, Cell (2019), https://doi.org/10.1016/j.cell.2019.03.032 .. REAGENT or RESOURCE SOURCE IDENTIFIER 2-Chlorotrityl chloride resin Advanced ChemTech Cat#SC5055 Z-VAD-FMK Enzo Life Sciences Cat#AXL-260-020-M001 RSL3 Cayman Chemical Cat#19288 Triisopropylsilane (98%) Acros Organics Cat#214920500 N,N’-diisopropylcarbodiimide Chem-Implex Int’l Cat#00110 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium 3-oxid hexafluorophosphate (HATU) Oakwood Chemical Cat#023926 Tat (GRKKRRQRRRPQ) Schwarze et al., 1999 N/A Tat-SelPep (GRKKRRQRRRPQUKUNLN) This paper N/A Tat-Cys (GRKKRRQRRRPQCKCNLN) This paper N/A Ndel New England Biosciences Cat#R0111S BamHl New England Biosciences Cat#R0136S HindIII New England Biosciences Cat#R0104S XBal New England Biosciences Cat#R0145T Critical Commercial Assays EZ-Magna ChIP Assay Kit Millipore Cat# 17-408 NucleoSpin RNA II Kit Clontech Cat#740955-250 TaqMan RNA to Ct 1-step kit Applied Biosystems Cat#4392656 MTT Assay Kit Promega Cat#G4100 Live/Dead Assay Kit Thermo Fisher Scientific Cat#L3224 Dual Luciferase Assay Kit Promega Cat#E1980 DeadEnd Fluorometric TUNEL system Promega Cat#G3250 MiRNeasy Kit QIAGEN Cat#217004 RiboZero Gold Kit Illumina Cat#MRZG1234 Quant-iT PicoGreen Assay Life Technologies Cat#p7589 Qiaquick Gel Extraction Kit QIAGEN Cat#28704 Quick T4 DNA Ligase Kit New England Biolabs Cat#M2200 Fast Ion Plasmid Maxi Kit IBI Scientific Cat# IB47125 Deposited Data RNA Sequencing of primary cortical neurons treated with selenium and HCA This paper https://www.ncbi.nlm.nih.gov/geo/, GEO: GSE126787 Raw western blots, WGCNA data, time lapse cell death videos, TUNEL staining, additional siRNA data, Z-VAD-FMK data and behavior scatterplots.



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